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KMID : 1020220170170030381
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Journal of Korean Society of Dental Hygiene
2017 Volume.17 No. 3 p.381 ~ p.394
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The concentration differences of dental caries induced organic acids which are produced after intake of sucrose and carbonated drinks
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Park Jung-Eun
Jang Jong-Hwa
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Abstract
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Objectives: This study aims to evaluate carbonated drinks induced dental caries with qualitative analysis and to compare with oral organic acids including lactate, acetate, propionate, formate, butyrate, pyruvate and valerate which cause caries when taking either 10% sucrose drinks or carbonated drinks.
Methods: Saliva was collected from six study subjects before and after (start, 5, 10, 30 minutes) taking water intake upon (A) 10% sucrose intake, (B) 10% sucrose intake, and (C) carbonated drink intake, then they were centrifuged at 1,200 rpm followed by removing bacteria and enzymes with syringe filtering, performing a qualitative analysis with HPLC conductivity detection (GP50 gradient pump, ED 50 detector) after saliva pre-treatment under isocratic 100 mM NaOH mobile phase.
Results: Higher risk of dental caries was evaluated in order of C>B>A, with the results of total oral organic acids` concentration, lactates of organic acids and organic acids produced after 5 minutes from the 3 types of drinks intake.
Conclusions: Carbonated beverages were estimated to develop higher dental caries induction than beverages containing 10% sucrose because of the high organic acid concentration in the mouth after its intake.
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KEYWORD
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Carbonated beverage, Dental caries, HPLC, Organic acid, Saliva
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